Skip To Content
Course listing icon for focusing on MALDI Imaging

Mass Spectrometry Imaging - Focus on MALDI Imaging

Time limit: 180 days

$250 Enroll

Full course description

Course Description

Mass spectrometry imaging (MSI) is a powerful technology in biomedical research, made available to the Johns Hopkins research community in the Applied Imaging Mass Spectrometry (AIMS) Core. The most widely used ionization technique used in overall MSI is matrix-assisted laser desorption/ionization (MALDI) imaging because of its high imaging speed and high spatial resolution. Spatially resolved MALDI imaging measurements are directly taken from frozen, formalin-fixed, or formalin-fixed paraffin-embedded (FFPE) tissue sections or cells deposited or grown on slides without destroying them.

MALDI imaging combines mass spectrometric analyses of biomolecules with simultaneous histological evaluation to analyze intact proteins, tryptic peptides (on-tissue tryptic digest), N-glycans (on-tissue PNGase digest), peptides, lipids, metabolites, drug molecules, and drug metabolites in a spatially resolved manner. This course will cover the basics of MSI and then focus on MALDI imaging, including sample preparation, on-tissue digests and derivatizations, and data analysis and consists of 8 online modules of 45-60 minutes each with embedded quizzes.


After successfully completing the course, learners will receive a certificate of completion from the Johns Hopkins School of Medicine.

Learning Prerequisites

There are no specific educational prerequisites; however, some basic knowledge of science, particularly biology, is recommended. You will need to have English language fluency.

Technical Prerequisites

The course will take place on the Canvas LMS ( You will need the ability to log on to the platform, preferably using Google Chrome, to complete the content, which will include videos, readings, and assignments. You may also need to take part in other activities on

  • Hardware requirements: computer; webcam; microphone and speakers or headset.
  • Software requirements: Google Chrome web browser; access to; Zoom web conferencing; Microsoft Office or similar; email

Course Lifecycle and Access

After registering, you will receive an email confirming your registration. You will be granted access to the course, which includes complete course information. Please check the course listing for additional information on course access dates. For synchronous courses, learners will have access to the Canvas course for the duration of the course. For asynchronous courses, learners will have access for 180 days after registration. 

Tuition Remission

This course may qualify for tuition remission. Please check for guidelines. If you are interested in using tuition remission, please email the OOE support email with your request.

Refund Policy and Timeline

  • Synchronous Courses
    • Refunds may be requested up to the start of the live activity, unless otherwise noted. All refund requests will incur a 10% processing fee.
  • Asynchronous Courses
    • Refunds are not allowed for active asynchronous courses.

Technical Support Statement

Please direct all technical support requests to the OOE support email. We will respond to requests within 24-48 hours. Requests received on weekends, overnights and holidays will be responded to on the next business day.

Accessibility Statement

JHU SOM is committed to providing all participants with the opportunity to pursue excellence in their learning endeavors. This includes providing disability related accommodations and services in accordance with the Americans with Disabilities Act (ADA) and our goal of delivering accessible, inclusive experiences. If you require accommodations to complete this course, please reach out to the  OOE support email. Please do not include any personal identifiable information such as social security number in any messages. 


JHU is not approved by Middle States for direct assessment credentials for academic credit. This course results in a certificate of completion. It will not show on any JHU transcripts and does not count toward an academic record.